Recombinant Mouse Anti-EBV gH/gL Antibody (E1D1)
CAT#: PABC-524
This product is a recombinant Mouse antibody that can recognize EBV gH/gL. E1D1 partially blocks membrane fusion with epithelial cells, but not B cells.
Published Data
Tested Data
ELISA
Figure 1 gHgL mutant surface expression and conformation was monitored using anti-gHgL E1D1, CL40 and CL59 monoclonal antibodies and HL800 polyclonal serum using a cell ELISA assay.
The results are expressed as % change in antibody binding relative to wt, with most antibodies showing no changes in binding to the mutants relative to the wt gHgL (values near zero represent wt binding). E1D1 binding is selectively lost for mutations in gl residues gl:L74, glL:176 and glL:Y131. n.d., not detected. A western blot of the gi N69 glycosylation mutants detected using a polyclonal anti-gHgL antibody (rabbit) is shown adjacent to the antibody binding data, with monoclonal anti-GAPDH blots shown as a loading control. The gel shows the expected shift in molecular weight of the gl mutants due to loss of glycan at the mutated NXS motif.
Sathiyamoorthy, K., Hu, Y. X., Möhl, B. S., Chen, J., Longnecker, R., & Jardetzky, T. S. (2016). Structural basis for Epstein–Barr virus host cell tropism mediated by gp42 and gHgL entry glycoproteins. Nature communications, 7, 13557.
Inhib
Figure 3 Inhibition of fusion activity by E1D1 mAb and Fab.
The x-axis indicates the amount of purified E1D1 antibody expressed as a final concentration of E1D1 mAb or Fab as present in a luciferase based cell–cell fusion assay using epithelial cells. No gH and gL plasmids transfected serves as the negative control denoted as neg. ctrl.
Sathiyamoorthy, K., Hu, Y. X., Möhl, B. S., Chen, J., Longnecker, R., & Jardetzky, T. S. (2016). Structural basis for Epstein–Barr virus host cell tropism mediated by gp42 and gHgL entry glycoproteins. Nature communications, 7, 13557.
❮
❯
SDS-PAGE
Figure 1 Recombinant Mouse Anti-EBV gH/gL Antibody (PABC-524) in SDS-PAGE
Lane M: Marker
Lane 1: PABC-524 in non-reduced condition.
Lane 2: PABC-524 in reduced condition.
HPLC
Figure 2 Recombinant Mouse Anti-EBV gH/gL Antibody (PABC-524) in HPLC
HPLC analysis of PABC-524 shows the purity is 99.57%.
❮
❯
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG2a, κ
- Specificity
- EBV gH/gL
- Species Reactivity
- EBV
- Clone
- E1D1
- Applications
- ELISA, FC, Neut, Inhib
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Flow Cytometry, Inhibition, Neutralization and Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- EBV gH/gL; Envelope glycoprotein H; Envelope glycoprotein L
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for PABC-524. Click the button above to contact us or submit your feedback about this product.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABC-524, RRID: AB_3111598)
Copy citation
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone E1D1"
- CAT
- Product Name
See other products for "EBV gH/gL"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-311 | Recombinant Human Anti-EBV gH/gL Antibody (AMMO1) | ELISA, BLI, Neut | Human IgG |
| PABC-419 | Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) | ELISA, Inhib, FC, Neut | Mouse IgG1 |
| PABC-523 | Mouse Anti-EBV gH/gL Recombinant Antibody (clone CL59) | ELISA, FC, Neut, Inhib | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-311 | Recombinant Human Anti-EBV gH/gL Antibody Fab Fragment (AMMO1) | ELISA, Neut | Human Fab |
| PFBC-419 | Recombinant Mouse Anti-EBV gH/gL Antibody Fab Fragment (CL40) | ELISA, FC, Neut | Mouse Fab |
| PFBC-523 | Recombinant Mouse Anti-EBV gH/gL Antibody Fab Fragment (CL59) | ELISA, FC, Neut | Mouse Fab |
| PFBC-524 | Recombinant Mouse Anti-EBV gH/gL Antibody Fab Fragment (E1D1) | ELISA, FC, Neut | Mouse Fab |
| PABC-523-F(E) | Mouse Anti-EBV gH/gL Recombinant Antibody (clone CL59); Fab Fragment | ELISA, FC, Neut, Inhib | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-311 | Recombinant Human Anti-EBV gH/gL Antibody scFv Fragment (AMMO1) | ELISA, Neut | Human scFv |
| PSBC-419 | Recombinant Mouse Anti-EBV gH/gL Antibody scFv Fragment (CL40) | ELISA, FC, Neut | Mouse scFv |
| PSBC-523 | Recombinant Mouse Anti-EBV gH/gL Antibody scFv Fragment (CL59) | ELISA, FC, Neut | Mouse scFv |
| PSBC-524 | Recombinant Mouse Anti-EBV gH/gL Antibody scFv Fragment (E1D1) | ELISA, FC, Neut | Mouse scFv |
| PABC-523-S(P) | Mouse Anti-EBV gH/gL Recombinant Antibody (clone CL59); scFv Fragment | ELISA, FC, Neut, Inhib | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-XY12 | Human Anti-EBV gH/gL Vaccinal Antibody (VS-0625-XY12) | ELISA, Neut | Human IgG |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, WB
Application: WB, ELISA, Neut, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, FuncS
-2.png)
Application: ELISA, WB
-2.png)
Application: ELISA
Application: ELISA, Inhib, FuncS
Application: ELISA, Inhib, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
